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anti-AES antibody product blog

Posted on 2015-07-11 21:11:20 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; AES; anti-AES antibody;
The AES aes (Catalog #MBS150869) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AES Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s AES can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF).
AES antibody can be used for the detection of AES by Western blot at 2 - 4 mug/mL. Antibody can also be used for immunocytochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the AES aes for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The AES aes product has the following accession number(s) (GI #39812019) (NCBI Accession #NP_945320) (Uniprot Accession #Q08117). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the AES Antibody with the following immunoassay(s):
Immunocytochemistry (ICC) (Immunocytochemistry of AES in 293 cells with AES antibody at 10 ug/mL.)
Immunocytochemistry (ICC) AES.

Immunofluorescence (IF) (Immunofluorescence of AES in 293 cells with AES antibody at 20 ug/mL.)
Immunofluorescence (IF) AES.

AES Antibody: Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment. Amino-terminal enhancer of split (AES) is a member of the Groucho/ transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis. It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. Interestingly, apoptosis of cells transfected with AES and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin.

Conjugate: Unconjugated
Immunogen: AES antibody was raised against a 16 amino acid synthetic peptide from near the amino-terminus of human AES.
Buffer: AES Antibody is supplied in PBS containing 0.02% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing AES are readily searchable from our website. Different antibodies against the same target such as AES may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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