|The ADORA2B adora2b (Catalog #MBS618019) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Adenosine A2b Receptor (A2b) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Adenosine A2b Receptor can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB).
Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 1:1000-1:5000 using ECL.
ELISA: 1:10,000-1:50,000 using 50-100ng A1R control peptide/well. Researchers should empirically determine the suitability of the ADORA2B adora2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The ADORA2B adora2b product has the following accession number(s) (GI #178150) (Uniprot Accession #P29275). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
The purine nucleoside adenosine modulates diverse physiological functions including induction of sedation, vasodilatation, suppression of cardiac rate and contractility, neurotransmitter release, inhibition of platelet aggregation and lipolysis. Adenosine released from cells interacts with membrane receptors (adenosine receptors, ARs). Based upon, biochemical and pharmacological criteria, ARs have been classified into A1, A2a, A2b, and A3. The high affinity receptor A1 inhibits adenyl cyclase, whereas low affinity receptor A2a stimulates the cyclase via G proteins. A2a receptor is believed to cause vasorelaxation in coronary artery. Various, ARs and their subtypes have recently been cloned from several species. ARs belong to the superfamily of G-protein coupled receptors and predicted to contain 7 transmembrane domains. The N-termini are predicted to be extracellular and the C-termini cytoplasmic. There is an overall 30% homology among the four ARs. ARs are distributed throughout the body. Gene location of various ARs: A1 (Chromosome 1; q.31.3-32.2); A2a (Chromosome 22); A2b (Chromosome 17; p.11.2-12); A3 (Chromos.1).
Immunogen: A 16 amino acid sequence corresponding to human brain A2bR gene (1). This region corresponds to the 2nd extracellular mid-region domain. Coupled to KLH. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ADORA2B are readily searchable from our website. Different antibodies against the same target such as ADORA2B may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.