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anti-ADIPOQ antibody product blog

Posted on 2014-11-25 12:55:03 by mybiosource_staff
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Tags: Antibody; Adiponectin; Monoclonal Antibody; anti-ADIPOQ antibody; ADIPOQ;
The ADIPOQ adipoq (Catalog #MBS219143) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s ADIPONECTIN can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Researchers should empirically determine the suitability of the ADIPOQ adipoq for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The ADIPOQ adipoq product has the following accession number(s) (GI #295317372) (NCBI Accession #NP_001171271.1) (Uniprot Accession #Q15848). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MOUSE ANTI HUMAN ADIPONECTIN with the following immunoassay(s):
Testing Data (Sandwich ELISA analysis of adiponectin binding using Mouse anti Human adiponectin as a capture reagent and biotinylated Mouse anti Human adiponectin (MBS214456) as a detection reagent with recombinant human adiponectin (MBS232423) as antigen for generation of the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader. A plasma sample diluted 1:4 (green) is shown)
Testing Data ADIPOQ.

Mouse anti Human Adiponectin antibody, clone Adn36 recognizes human adiponectin, also known as Acrp30, 30 kDa adipocyte complement-related protein, Adipocyte, C1q and collagen domain-containing protein or Gelatin-binding protein. Adiponectin is a 244 amino acid major adipokine secreted into the bloodstream from adipose tissue to modulate metabolism, including glucose regulation and fatty acid catabolism (UniProt: Q15848). Adiponectin circulates as three distinct oligomeric forms, trimers (LMW), hexamers (MMW) and dodecamers-octodecamers (HMW) with distinct functions in various target tissues (Wang et al. 2008). Adiponectin is involved in regulation of circulating glucose levels and in regulation of insulin sensitivity. Concentrations of adiponectin are reduced in obesity associated metabolic and vascular disorders (Fasshauer et al. 2004) in particular lower circulating levels of HMW adiponectin correleates with the appearance of type II diabetes and acts as a predictor for this condition (Zhu et al. 2010). Mouse anti human adiponectin antibody, clone Adn36 has been used successfully in the development of a sensitive sandwich ELISA in conjunction with mouse anti human adiponectin antibody, clone 5H7 as a detection reagent for the measurement of circulating levels of adiponectin in human serum.

Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant. Buffer Solution: Phosphate buffered saline
Target Species: Human. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ADIPOQ are readily searchable from our website. Different antibodies against the same target such as ADIPOQ may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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