|The ADIPOQ adipoq (Catalog #MBS200018) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Adiponectin antibody (clone 1G12) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s Adiponectin can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB) (Recombinant protein,Cell lysate).
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:250 ~ 1,000. Recommended starting dilution is 1:500. Please note that this antibody (1G12) has the specificity against globular domain of adiponectin. Researchers should empirically determine the suitability of the ADIPOQ adipoq for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The ADIPOQ adipoq product has the following accession number(s) (GI #4757760) (NCBI Accession #NP_004788). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Adiponectin antibody (clone 1G12) with the following immunoassay(s):
Western Blot (WB) (The recombinant proteins and mouse liver lysates were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human adiponectin antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Arrows indicate the oligomer and monomer of mAcrp30 protein in mouse liver. * gAcrp30 : globular domain of adiponectin )
Human Adiponectin, also referred to as AdipoQ, Acrp30, apm-1 or GBP28, is a secreted protein expressed exclusively in differentiated adipocyte(adipokine). Adiponectin contains a modular structure comprising an N-terminal collagenous domain followed by a C-terminal globular domain(gAcrp30). Adiponectin plays a role in various physiological processes such as energy homeostasis and obesity. Plasma levels of adiponectin are reduced in obese humans, and decreased levels are associated with insulin resistance and hyperinsulinemia.
Antigen Species: Human
Clone: Anti-human adiponectin mAb, clone 1G12, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Acrp30 protein.
Immunogen: Recombinant human adiponectin (15-244aa) purified from E Coli. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ADIPOQ are readily searchable from our website. Different antibodies against the same target such as ADIPOQ may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.