anti-ACTN1 antibody product blog
Tags: Antibody; ACTN1; anti-ACTN1 antibody; Polyclonal Antibody; Actinin, alpha 1;
The ACTN1 actn1 (Catalog #MBS623096) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Actinin, alpha 1 (Alpha Actinin 1, alpha-Actinin-1, ACTN1, alpha-Actinin Cytoskeletal Isoform, F-actin Cross-linking Protein, FLJ40884, FLJ54432, Non-muscle alpha-Actinin-1) reacts with Hamster, Human, Monkey, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Actinin, alpha 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF).Suitable for use in Immunofluorescence and Western Blot.
Dilution: Immunofluorescence (IC): 1:25
Western Blot: 1:1000. Researchers should empirically determine the suitability of the ACTN1 actn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The ACTN1 actn1 product has the following accession number(s) (GI #30583253) (Uniprot Accession #P12814). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Actinin, alpha 1 (Alpha Actinin 1, alpha-Actinin-1, ACTN1, alpha-Actinin Cytoskeletal Isoform, F-actin Cross-linking Protein, FLJ40884, FLJ54432, Non-muscle alpha-Actinin-1) with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of extracts from NIH/3T3, C6, CHO and COS cells using MBS623096.)
Immunofluorescence (IF) (Confocal immunofluorescent analysis of NIH/3T3 cells using MBS623096 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).)
a-actinin belongs to the spectrin family of cytoskeletal proteins. It was first recognized as an actin cross-linking protein, forming an antiparallel homodimer with an actin binding head at the amino terminus of each monomer. More recently, a-actinin has been shown to interact with a large number of proteins involved in signaling to the cytoskeleton including those involved in cellular adhesion, migration and immune cell targeting. (1). The interaction of a-actinin with ICAM5 (intercellular adhesion molecule-5) helps to promote neurite outgrowth (2). In osteoblasts, interaction of a-actinin with integrins stabilizes focal adhesions and may protect cells from apoptosis (3). Isoforms 1 and 4 of a-actinin, which are non-muscle isoforms, are present in stress fibers, sites of adhesion and intercellular contacts, filopodia and lamellipodia. The muscle isoforms 2 and 3 localize to the Z-discs of striated muscle and to dense bodies and plaques in smooth muscle (1).
Immunogen: Synthetic peptide corresponding to carboxy-terminal residues of human alpha-actinin-1, conjugated to KLH (P12814). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ACTN1 are readily searchable from our website. Different antibodies against the same target such as ACTN1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.