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anti-Actin, Smooth Muscle antibody product blog

Posted on 2013-10-16 00:50:38 by mybiosource_staff
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Tags: Antibody; Actin, Smooth Muscle; anti-Actin, Smooth Muscle antibody;
The Actin, Smooth Muscle n/a (Catalog #MBS462003) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti SMA antibody (Smooth Muscle Actin) reacts with Hu, MS, Rt, Dg, Bv, Sh, Gp, Fg and may cross-react with other species as described in the data sheet. MyBioSource\'s Actin, Smooth Muscle can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation, Immunofluorescence.
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 2-5 ug/ml
Flow Cytometry: 10-20 ug per 10^6 cells

Positive Control: most cells
Cellular Location: Cytoplasmic

Optimal dilutions should be determined by researchers for the specific applications. Researchers should empirically determine the suitability of the Actin, Smooth Muscle n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rabbit anti SMA antibody (Smooth Muscle Actin) with the following immunoassay(s):
Testing Data
Testing Data Actin, Smooth Muscle.

The Actin protein a component of the eukaryotic cytoskeleton is highly conserved between multiple species. It is involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. The levels of the Actin protein may vary between different cell types. But are consistent within a given cell type, and for this reason, Actin protein levels are considered an internal control for protein loading.

Antigen Preparation: A 10aa synthetic peptide corresponding to N-term of human Actin protein, smooth muscle specific. This sequence is identical in human, mouse, rat, bovine and chicken. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Actin, Smooth Muscle are readily searchable from our website. Different antibodies against the same target such as Actin, Smooth Muscle may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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