anti-ACTB antibody product blog
Tags: Antibody; ACTB; anti-ACTB antibody; beta-actin; Polyclonal Antibody;
The ACTB actb (Catalog #MBS151223) is an Antibody produced from Chicken and is intended for research purposes only. The product is available for immediate purchase. The Beta-actin Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Beta-actin can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB).Beta-actin antibody can be used for the detection of beta-actin by Western blot at 1 - 2 mug/mL. Researchers should empirically determine the suitability of the ACTB actb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The ACTB actb product has the following accession number(s) (GI #12803203) (NCBI Accession #AAH02409) (Uniprot Accession #P60709). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Beta-actin Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of actin in HeLa cell lysate with beta-actin antibody at (A) 1 and (B) 2 μg/mL.)
Beta-actin Antibody: Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, beta-actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
Conjugate: Unconjugated
Immunogen: Beta-actin antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human beta-actin.
Buffer: Beta-actin Antibody is supplied in PBS containing 0.02% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ACTB are readily searchable from our website. Different antibodies against the same target such as ACTB may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Blood, Bone, Brain, Connective Tissue, Embryonic Tissue, Heart, Kidney, Liver, Lung, Muscle tissues are correlated with this protein. Brain Diseases, Carcinoma, Disease Models, Animal, Heart Diseases, Inflammation, Kidney Diseases, Liver Diseases, Liver Neoplasms, Lung Diseases, Necrosis are some of the diseases may be linked to Beta-actin Antibody.