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anti-ACHE antibody product blog

Posted on 2015-02-06 10:54:16 by mybiosource_staff
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Tags: Antibody; ACHE; Polyclonal Antibody; anti-ACHE antibody;
The ACHE ache (Catalog #MBS420039) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-ACHE Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Cow and may cross-react with other species as described in the data sheet. MyBioSource\'s ACHE can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB).
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 67kDa band observed in Human Brain (Cerebellum and Hippocampus) lysates (calculated MW of 67kDa according to NP_000656.1). Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the ACHE ache for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The ACHE ache product has the following accession number(s) (GI #4557239) (NCBI Accession #NP_000656.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Goat anti-ACHE Antibody with the following immunoassay(s):
Western Blot (WB) ((0.3ug/ml) staining of Human Brain (Cerebellum) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB) ACHE.

Immunogen: Peptide with sequence QFDHYSKQDRCSDL, from the C Terminus of the protein sequence according to NP_000656.1.
Epitope: C Terminus. Note: This antibody is expected to recognise isoform NP_000656 only (the ubiquitously expressed, hydrophillic form). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ACHE are readily searchable from our website. Different antibodies against the same target such as ACHE may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Brain Diseases, Edema, Hypertension, Inflammation, Kidney Diseases, Liver Diseases, Necrosis, Nervous System Diseases, Peripheral Nervous System Diseases, Weight Loss are some of the diseases may be linked to Goat anti-ACHE Antibody. Blood, Brain, Heart, Intestine, Kidney, Liver, Lung, Mouth, Muscle, Nerve tissues are correlated with this protein. The following patways have been known to be associated with this gene. ACHE also interacts with the following gene(s): APP, CHAT, COLQ, JUN, SLC44A1.
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