anti-ACE2 antibody product blog
Tags: Antibody; ACE2; Polyclonal Antibody; anti-ACE2 antibody;
The ACE2 ace2 (Catalog #MBS628626) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ACE2, ID (Angiotensin-converting Enzyme 2, ACE-related Carboxypeptidase, Angiotensin-converting Enzyme Homolog, ACEH, Metalloprotease MPROT15, Processed Angiotensin-converting Enzyme 2, UNQ868/PRO1885) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s ACE2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC).Western Blot: 1:1000
Immunohistochemistry (FFPE): 1:50-1:100
Other applications have not been tested. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the ACE2 ace2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The ACE2 ace2 product has the following accession number(s) (GI #11225609) (NCBI Accession #NP_068576.1) (Uniprot Accession #Q9BYF1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the ACE2, ID (Angiotensin-converting Enzyme 2, ACE-related Carboxypeptidase, Angiotensin-converting Enzyme Homolog, ACEH, Metalloprotease MPROT15, Processed Angiotensin-converting Enzyme 2, UNQ868/PRO1885) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of human kidney tissue lysate (35ug) using MBS628626 (1:1000). A goat anti-rabbit IgG H&L (HRP) at 1:5000 was used as the secondary antibody.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC) (Immunohistochemistry analysis in formalin-fixed paraffin-embedded human kidney tissue MBS628626 followed by peroxidase-conjugated to secondary antibody and DAB staining. This data demostrates the use of MBS628626 for immunohistochemistry)
ACE2 cDNA encodes a deduced 805aa protein containing a potential 17aa N-terminal signal peptide and a putative 22aa C-terminal membrane anchor. It also possesses a zinc metalloprotease consensus sequence and a conserved glutamine residue that may function as a third zinc ligand. ACE2 is expressed predominantly in vascular endothelial cells of the heart and kidney. ACE converts angiotensin I to angiotensin II, ACE2 converts angiotensin I to angiotensin 1-9, which has 9aa. Angiotensin II is a potent blood vessel constrictor, while angiotensin 1-9 does not impact blood vessels but is cleaved by ACE to a shorter peptide, angiotensin 1-7, which is a blood vessel dilator. Spike (S) proteins of coronaviruses, including the SARS coronavirus, bind with cellular receptors to mediate infection of target cells. ACE2 binds the S1 domain of the SARS coronavirus S protein. SARS coronavirus replicates efficiently on ACE2-transfected but not mock-transfected 293T cells. Anti-ACE2 but not anti-ACE1 antibody blocks viral replication on Vero E6 cells. It has been proposed that ACE2 is a functional receptor for SARS coronavirus.