anti-ABCB11 antibody product blog
Tags: Antibody; Monoclonal Antibody; ABCB11; anti-ABCB11 antibody; BSEP;
The ABCB11 abcb11 (Catalog #MBS603822) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The BSEP (Bile Salt Export Pump, SPGP, Sister of P-glycoprotein, ABCB11) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s BSEP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC).Suitable for use in Immunocytochemistry and Western Blot.
Dilution: Western Blot: 1:50-1:200
Immunocytochemistry: Cytospin preparations. Researchers should empirically determine the suitability of the ABCB11 abcb11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The ABCB11 abcb11 product has the following accession number(s) (GI #21536378) (NCBI Accession #NP_003733.2) (Uniprot Accession #O95342). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the BSEP (Bile Salt Export Pump, SPGP, Sister of P-glycoprotein, ABCB11) with the following immunoassay(s):
Western Blot (WB) (WB staining of crude plasma membrane purified from human liver tissue collected by fine needle biopsy. Protein load: 40ug. MBS603822 dilutions: 1:200, 1:150, 1:100, and 1:50 (lanes 1-4, respectively).)
Western Blot (WB) (Western blot using MBS603822 in crude membranes isolated from human liver (hL), human trophocytes (hT) and human placenta (hP).)
BSEP is involved in the ATP-dependent secretion of bile salts into the canaliculus of hepatocytes. It is expressed predominatly, if not exclusively, in the liver, where it is further localized to the canilicular microvilli and to subcanilicular vesicles fo the hepatocytes. Structurally, BSEP is a multifunctional polypeptide with two homologus halves, each containing a hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain. Defects in BSEP are the cause of progressive familial intrahepatic cholestasis 2 (PFIC2). PFIC2 is an inherited liver disease of childhood which is characterized by cholestasis and normal serum gamma-glutamyltransferase activity. Defects in BSEP are also found in cases of chronic intrahepatic cholestasis without obvious familial history of chronic liver disease.
Immunogen: Synthetic peptide corresponding to aa705-718, Y705LVHEPPLAVVDHK718 of human BSEP (bile salt export pump). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ABCB11 are readily searchable from our website. Different antibodies against the same target such as ABCB11 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.