|The a2-Macroglobulin n/a (Catalog #MBS617996) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The a2-Macroglobulin reacts with Canine, Human, Porcine, Rabbit and may cross-react with other species as described in the data sheet. MyBioSource\'s a2-Macroglobulin can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA).
Suitable for use in ELISA, Immunoprecipitation and Immunoelectrophoresis. Researchers should empirically determine the suitability of the a2-Macroglobulin n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
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Alpha2 Macroglobulin (a-2M) is a large proteinase inhibitor molecule of 718kD, consisting of 4 identical subunits of 185,000 each. It is produced in hepatocytes and macrophages, and generally found in plasma concentrations of 2uM in adults, and up to 6uM in children. a-2M inhibits most enzymes from the serine, metallo, cysteine and aspartate subclasses. It is classed with proteins that include pregnancy zone protein (PZP) and the complement proteins C3, C4 and C5. These proteins contain regions of conserved sequence as well as one or more internal b-cysteinyl-g-glutamyl thiolester bonds. In a-2M, these regions are susceptible to cleavage by enzymes or by nucleophilic compounds such as methylamine or ammonium ions. It is thought that enzymatic cleavage of a bait region within the a-2M molecule causes a conformational change, which then traps and/or covalently binds the enzyme (1,2). The active site of the trapped enzyme is usually still intact and able to cleave small substrates, but is inaccessible to larger natural substrates. The conformational change also exposes receptor-binding regions within the molecule, which may be important in the clearance of a-2M enzyme complexes from the circulation. It is thought that the main role of a-2M in vivo is "backup" inhibitor and scavenger of proteinases in blood and in tissues (3,4). It has also been reported to participate in other physiological processes, including regulation of immune function (1,2).
Immunogen: Human Macroglobulin, alpha2 purified from plasma. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing a2-Macroglobulin are readily searchable from our website. Different antibodies against the same target such as a2-Macroglobulin may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.