|The a-Dystroglycan n/a (Catalog #MBS603708) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The a-Dystroglycan (Cranin) reacts with Canine, Guinea Pig, Human, Mouse, Rabbit, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s a-Dystroglycan can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC).
Suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation and Western Blot.
Dilution: Immunohistochemistry (Frozen): 1:100 detected a-Dystroglycan in frozen rabbit skeletal muscle sections fixed with 50% ethanol and 50% acetic acid.
Immunoprecipitation: 10ul immunprecipitates dystroglycan from 750ug of C2C12 cell lysate.
Western Blot: 1:100-1:1000. Detects a-Dystroglycan in C2C12 cell lysate. Previous lot detected Dystroglycan in rabbit skeletal muscle. Researchers should empirically determine the suitability of the a-Dystroglycan n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
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Dystroglycan is one of the dystrophin-associated glycoproteins, which is encoded by a 5.5kb transcript in Homo sapiens by chromosome 3. There are two exons that are separated by a large intron. The spliced exons codes for a protein product is finally cleaved into two non-covalently associated subunits, alpha (N-terminal) and beta (C-terminal). In general, aberrant expression of dystrophin-associated protein complex underlies the pathogenesis of Duchenne muscular dystrophy, Becker muscular dystrophy and severe childhood autosomal recessive muscular dystrophy. Interestingly, no genetic disease has been described for either alpha or beta-dystroglycan. Dystroglycan is widely distributed in non-muscle tissues as well as in muscle tissues. During epithelial morphogenesis of kidney, the dystroglycan complex is shown to act as a receptor for the basement membrane. Dystroglycan expression in Mus musculus brain and neural retina has also been reported. However, the physiological role of dystroglycan in non-muscle tissues has remained unclear.
Immunogen: Rabbit skeletal muscle membrane preparation to alpha Dystroglycan. Positive Control: Mouse, rat and human skeletal muscle tissue extracts or DU145 (human prostate carcinoma tumor) cell lysate. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing a-Dystroglycan are readily searchable from our website. Different antibodies against the same target such as a-Dystroglycan may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.