anti-6-HIS antibody product blog
Tags: Antibody; Polyclonal Antibody; 6-HIS; anti-6-HIS antibody;
The 6-HIS n/a (Catalog #MBS560109) is an Antibody produced from Chicken and is intended for research purposes only. The product is available for immediate purchase.ELISA: Suggested starting dilution 0.2 ug/mL. Western Blot: Suggested starting dilution is 1.0 ug/mL. Researchers should empirically determine the suitability of the 6-HIS n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Chickens were immunized with 6xHIS peptide conjugated to a carrier protein. Eggs were collected and an IgY fraction was prepared. The resulting fraction was passed over an antigen containing immunoabsorbant from which the anti-HIS tag antibodies were immunoaffinity purified. Purification was followed by conjugation with horseradish peroxidase (HRP).
Buffer: 50 mM HEPES - Saline with Stabilizer. Preservative: 0.002% Thimerosal
Procedure: Chickens were immunized with HHHHHH conjugated with KLH. After multiple immunizations in Freund\'s adjuvant, eggs were collected. Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and the antibodies were conjugated with HRP (Horseradish Peroxidase). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing 6-HIS are readily searchable from our website. Different antibodies against the same target such as 6-HIS may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.