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anti-4E-BP1 antibody product blog

Posted on 2014-01-04 20:11:10 by mybiosource_staff
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Tags: Antibody; anti-4E-BP1 antibody; 4E-BP1; Monoclonal Antibody;
The 4E-BP1 eif4ebp1 (Catalog #MBS850256) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The 4E-BP1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s 4E-BP1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA).
IF:1/200 - 1/1000, IHC:1/200 - 1/1000, ELISA: Propose dilution 1/10000
Not yet tested in other applications. Determining optimal working dilutions by titration test. Researchers should empirically determine the suitability of the 4E-BP1 eif4ebp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The 4E-BP1 eif4ebp1 product has the following accession number(s) (GI #4758258) (NCBI Accession #NP_004086.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the 4E-BP1 Antibody with the following immunoassay(s):
Testing Data (Figure 1. Western blot analysis using 4E-BP1 mouse mAb against truncated 4E-BP1 recombinant protein (1).)
Testing Data 4E-BP1.

Testing Data #2 (Figure 2. Immunohistochemical analysis of paraffin-embedded human pancreas carcinoma (A), esophagus carcinoma tissue (B) and ovary tumor tissue, showing cytoplasmic and membrane localization using 4E-BP1 mouse mAb with DAB staining.)
Testing Data #2 4E-BP1.

Background: 4E-BP1(eukaryotic translation Initiation Factor 4E Binding Protein 1),also called ELF4EBP1/BP-1/PHAS-I,which is located on chromosome 8p12, with 118-amino acid protein (about 13kDa). Binding of eIF4EBP1 to eIF4E is reversible and is dependent on the phosphorylation status of eIF4EBP1. Non phosphorylated eIF4EBP1 will bind strongly to eIF4E while(24kDa), the phosphorylated form will not. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating eIF4EBP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the eIF4EBP1-eIF4E interaction.

Immunogen: Purified recombinant fragment of 4EBP1 expressed in E. Coli.
Species: Mouse IgG1. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing 4E-BP1 are readily searchable from our website. Different antibodies against the same target such as 4E-BP1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. 4E-BP1 also interacts with the following gene(s): AKT1, ATM, EIF4E, EIF4G1, MLST8, MTOR, RHEB, RPS6KB1, RPTOR.
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