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anti-4-Hydroxynonenal antibody product blog

Posted on 2013-11-19 13:06:29 by mybiosource_staff
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Tags: Antibody; 4-Hydroxynonenal; Polyclonal Antibody; anti-4-HYDROXYNONENAL antibody;
The 4-Hydroxynonenal n/a (Catalog #MBS316152) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s 4-Hydroxynonenal can be used in a range of immunoassay formats including, but not limited to, EIA/ELISA, Immunohistochemistry.
ELISA using immunizing peptide: >1:10,000. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. Researchers should empirically determine the suitability of the 4-Hydroxynonenal n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Goat anti 4-Hydroxynonenal with the following immunoassay(s):
Immunohistochemistry (Customer reported this item can be applied to mouse tissue in IHC paraffin. The photos below,which appear positive for fatty liver and negative for another normal one.
1. citrate buffer pH 6 100 degree C for 20 min. (RE7113 Leica Novocastra)
2. primary Ab. 200 X for 30 min at Room Temperature
3. rabbit x goat conjugate HRP 500 X for 20 min at RT. Rabbit anti-Goat IgG antibody HRP Conjugated
4. anti Rabbit polymer HRP(RTU) for 10min at RT (RE7112 Leica Novocastra)
5. DAB for 3 min. (RE7190K Leica Novocastra))
Immunohistochemistry 4-Hydroxynonenal.

Immunogen: 4-Hydroxynonenal conjugated Bovine Serum Albumin (Catalog #MBS318058). Buffer: Not applicable
Preservative: 0.1% Sodium azide
Important Note: Centrifuge before opening to ensure complete recovery of vial contents.
Warnings: This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1 - 1.0 %. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing 4-Hydroxynonenal are readily searchable from our website. Different antibodies against the same target such as 4-Hydroxynonenal may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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