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anti-14-3-3 TAU/THETA antibody product blog

Posted on 2013-09-16 12:55:26 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; 14-3-3 TAU/THETA; anti-14-3-3 TAU/THETA antibody;
The 14-3-3 TAU/THETA n/a (Catalog #MBS222335) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RABBIT ANTI 14-3-3 TAU/THETA reacts with Bovine, Chicken, Human, Mammals (Expected from Sequence), Mouse, Rabbit, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s 14-3-3 TAU/THETA can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistology Paraffin*, Western Blot (WB).
Western Blot: This item detects a band of approximately 30kDa in HEK293 cell lysates.
Immunohistology - Paraffin: Maximum Dilution: 1/60; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Western Blotting: Maximum Dilution: 1/3000. Researchers should empirically determine the suitability of the 14-3-3 TAU/THETA n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Rabbit anti 14-3-3 τ/θ antibody recognizes 14-3-3 tau/theta in all mammals. It is a member of the 14-3-3 family which consists of 30 kDa proteins involved in multiple cell signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preferences for it\'s different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein. 14-3-3 tau/theta is primarily expressed in T-cells although it occurs at lower levels in the brain, heart, pancreas placenta and kidneys. It plays a role in cell cycle progression via interaction with p27(Kip1). Along with COPI, the theta isoform binds the GB1 RSR sequence involved in protein trafficking. The isoform forms a complex with Hsp60 and cellular prion protein which may be involved in prion diseases. Elevated levels of 14-3-3 tau/theta are observed in amyotrophic lateral sclerosis. 14-3-3 tau/theta also binds BCR/Abl, bacterial effector protein Tir, yes-associated protein and the FSH receptor. Rabbit anti 14-3-3 τ/θ antibody may not react with recombinant proteins that are not N-acetylated.

14-3-3 TAU/THETA n/a

Perservative Stabilisers: 0.09% Sodium Azide (NaN3). Immunogen: Synthetic peptide corresponding to acetylated N-terminal sequence of sheep 14-3-3 tau/theta. Peptide sequence: Ac.MEKTELIQKAC
Histology Positive Control Tissue: Normal tonsil tissue.
Antiserum Preparation: Antisera to sheep 14-3-3 tau/theta were raised by repeated immunisations of rabbit with highly purified antigen.
Target Species: Sheep. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing 14-3-3 TAU/THETA are readily searchable from our website. Different antibodies against the same target such as 14-3-3 TAU/THETA may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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