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Ang-2 recombinant protein product blog

Posted on 2013-10-06 21:05:30 by mybiosource_staff
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Tags: Recombinant Protein; Angiopoietin-2; Ang-2; Ang-2 recombinant protein;
The Ang-2 angpt2 (Catalog #MBS400087) is a Recombinant Protein produced from Insect Cells and is intended for research purposes only. The product is available for immediate purchase.

The Ang-2 angpt2 product has the following accession number(s) (GI #169646754) (NCBI Accession #NP_001112360.1) (Uniprot Accession #O15123). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
The N-terminal sequence is that of myc.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Our knowledge of the role of proteins in cellular processes is continually evolving. Most proteins, including angiopoietin-2 are typically involved in one or more signaling pathways or biological processes. Professionally manufactured recombinant proteins are increasingly becoming essential and commonplace tools for elucidating new knowledge about the role of proteins in both health and disease.

Ang-2 angpt2

Solubility: It is recommended to reconstitute the lyophilized Angiopoitin-2 in sterile 18Momega-cm H2O not less than 100ug.
Protein Content: Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.40 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of ANG-2 Human as a Reference Standard.
Dimers and Aggregates: Less than 5%. Formulation: Ang-2 Human is formulated from a solution containing TBS & 0.05% CHAPS.
Biological Activity: The biological activity of Angiopoietin-2 was determined by the induction of endothelial cell sprouting as described in Korff et al., 2001. Bone Marrow, Brain, Embryonic Tissue, Eye, Kidney, Liver, Lung, Muscle, Placenta, Vascular tissues are correlated with this protein. Ang-2 also interacts with the following gene(s): ANGPT1, ETS1, FGF2, FLT1, FLT4, FOXO1, KDR, TEK, TIE1, VEGFA. The following patways have been known to be associated with this gene. Cardiovascular Diseases, Disease Models, Animal, Heart Diseases, Hypertension, Inflammation, Kidney Diseases, Liver Diseases, Liver Neoplasms, Necrosis, Neoplasms, Experimental are some of the diseases may be linked to Human angiopoietin-2 (Ang-2).
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