ANA Screen IgG elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; ANA Screen IgG; ANA Screen IgG elisa kit;
The Human ANA Screen IgG n/a (Catalog #MBS580075) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS580075 ELISA Kit recognizes Human ANA Screen IgG.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Intended Uses: ANA Screen ELISA test system is an enzyme-linked immunosorbent assay (ELISA) for the detection of IgG class antibodies to ANA in human serum or plasma.
Principle of the Assay: Diluted patient serum is added to wells coated with purified nuclear antigens. ANA IgG specific antibody, if present, binds to the antigen. All unbound materials are washed away and the enzyme conjugate is added to bind to the antibody-antigen complex, if present. Excess enzyme conjugate is washed off and substrate is added. The plate is incubated to allow the hydrolysis of the substrate by the enzyme. The intensity of the color generated is proportionai to the amount of IgG specific antibody in the sample.
Samples: Human serum or plasma. ANA Screen IgG ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.