Alw44I restriction enzyme product blog
Tags: Restriction Enzyme; Alw44I; Alw44I restriction enzyme;
The Alw44I n/a (Catalog #MBS638487) is a Restriction Enzyme and is intended for research purposes only. The product is available for immediate purchase.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given restriction enzyme. We\'ve tested the Alw44I (ApaLI) with the following immunoassay(s):
Testing Data ()
5\'-G^T G C A C-3\'.
Supplied With: R1625-Restriction Enzyme Buffer A (double digests): 1X Buffer composition-Supplied as a liquid containing 33mM Tris-acetate pH 7.9, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA. Incubate at 37 degree C. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide occurred during incubation with 10 units of Alw44l for 4 hours.
Lambda: 4
Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with Alw44I more than 90% of the DNA fragments can be ligated at a 5\'-termini concentration of 0.03uM. More than 95% of these can be recut.
pBR322: 3
PhiX174: 1
pTZ19R/U: 2
pUC18/19: 3
pUC57: 3
Storage Buffer: Supplied as a liquid in 10mM Tris-HCl pH 7.5, 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA, 50% glycerol.
Thermal Inactivation: Enzyme is inactivated by incubation at 65 degree C for 20 minutes.
Unit Definition: One unit is defined as the amount of Alw44l required to digest 1ug of lambda DNA-Smal fragments in 1 hour at 37ºC in 50ul of assay buffer.