Alw21I restriction enzyme product blog
Tags: Restriction Enzyme; Alw21I; Alw21I restriction enzyme;
The Alw21I n/a (Catalog #MBS638454) is a Restriction Enzyme produced from Acinetobacter lwoffi RFL21 and is intended for research purposes only. The product is available for immediate purchase.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given restriction enzyme. We\'ve tested the Alw21I (HgiAI) with the following immunoassay(s):
Testing Data ()
5\' - G W G C W^C - 3\'.
Supplied With: R1625: Restriction Enzyme Buffer A, 10X: Supplied as a liquid in 33mM Tris-acetate, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA pH 7.9 at 37 degree C when diluted to 1X. Note: 1X conc. yields 20-50% enyzme activity. 2X conc. yields 50-100% enzyme activity. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of Alw21I for 4 hours.
Lambda: 28
M13mp18/19: 3
pBR322: 8
PhiX174: 3
pTZ19R/U: 4
pUC18/19: 5
pUC57: 5
Unit Definition: One unit is defined as the amount of Alw21I required to digest 1ug of lambda DNA in 1 hour at 37 degree C in 50ul of assay buffer.