ALB elisa kit product blog
Tags: ELISA Kit; Albumin; ALB elisa kit; ALB; Dog ELISA Kit;
The Dog ALB alb (Catalog #MBS705539) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS705539 ELISA Kit recognizes Dog ALB.The ALB alb product has the following accession number(s) (GI #55742764) (NCBI Accession #NP_001003026.1) (Uniprot Accession #P49822). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Principle of the Assay: This assay employs the direct competitive inhibition enzyme immunoassay technique. Antibody specific for Alb has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with biotin-conjugated Alb. A competitive inhibition reaction is launched between Alb (Standards or samples) and biotin-conjugated Alb with the pre-coated Alb antibody. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of Alb bound in the initial step. The color development is stopped and the intensity of the color is measured.
Samples: Serum, plasma, tissue homogenates.
Assay Type: Competitive
Detection Range: 0.273ug/ml - 35ug/ml.
Sensitivity: 0.226 ug/ml. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess. Dog Albumin, Alb ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Blood, Heart, Kidney, Liver, Lung, Mixed, Spleen tissues are correlated with this protein. The following patways have been known to be associated with this gene. Brain Diseases, Cardiovascular Diseases, Disease Models, Animal, Fibrosis, Heart Diseases, Kidney Diseases, Kidney Failure, Chronic, Liver Cirrhosis, Proteinuria, Respiratory Tract Diseases are some of the diseases may be linked to Dog Albumin, Alb ELISA Kit. ALB also interacts with the following gene(s): AHSG, AMBP, FN1, HPX, INS.