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ADAM28 recombinant protein product blog

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Posted on 2013-09-15 11:51:15 by mybiosource_staff
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Tags: Recombinant Protein; ADAM28; A Disintegrin And Metalloprotease 28 (ADAM28); ADAM28 recombinant protein;
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The ADAM28 adam28 (Catalog #MBS2010562) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s A Disintegrin And Metalloprotease 28 (ADAM28) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), CoIP, ReporterAssays, Purification, Amine Reactive Labeling. Researchers should empirically determine the suitability of the ADAM28 adam28 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The ADAM28 adam28 product has the following accession number(s) (GI #98985828) (NCBI Accession #NP_055080.2) (Uniprot Accession #Q9UKQ2). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSG SEF-EVADKS CYNRNEGGSK YGYCRRVDDT LIPCKANDTM CGKLFCQGGS DNLPWKGRIV TFLTCKTFDP EDTSQEIGMV ANGTKCGDNK VCINAECVDI EKAYKSTNCS SKCKGHAVCD HELQCQCEEG WIPPDCDDSS VVFHFSIVVG VLFPMAVIFV VVAMVIRHQS SREKQKKDQR PLSTTGTRPH KQKRKPQMVK AVQPQEMSQM KPHVYDLPVE GNEPPASFHK DTNALPPTVF KDNPVSTPKD SNPK.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given recombinant protein. We\'ve tested the Recombinant A Disintegrin And Metalloprotease 28 (ADAM28) with the following immunoassay(s):
SDS-PAGE
SDS-PAGE ADAM28.

Test Data
Test Data ADAM28.

Organism: Homo sapiens (Human)
Expression System: Prokaryotic expression
Residues: Glu525-Lys774 (Accession # Q9UKQ2) with N-terminal His-Tag
Tissue Specificity: Spleen, Stomach, Intestine, Brain.
Subcellular Location: Cell membrane; Single-pass type I membrane protein.
Traits: Freeze-dried powder
Predicted isoelectric point: 7.5
Predicted Molecular Mass: 29.3kDa
Accurate Molecular Mass: 34kDa as determined by SDS-PAGE reducing conditions.
Phenomenon explanation: The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc. . Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex. Bladder, Blood, Connective Tissue, Embryonic Tissue, Kidney, Lung, Lymph, Lymph Node, Mouth, Testis tissues are correlated with this protein. ADAM28 also interacts with the following gene(s): ADAMDEC1, ITGA4, ITGB1, LGI1. Adenocarcinoma, Atherosclerosis, Breast Neoplasms, Congenital Abnormalities, Disease Models, Animal, Inflammation, Kidney Diseases, Liver Diseases, Lymphoma, Neoplasms, Experimental are some of the diseases may be linked to Recombinant A Disintegrin And Metalloprotease 28 (ADAM28).
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