ADAM10 elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; A Disintegrin and Metalloprotease 10; ADAM10 elisa kit; ADAM10;
The Human ADAM10 adam10 (Catalog #MBS726961) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS726961 ELISA Kit recognizes Human ADAM10.The ADAM10 adam10 product has the following accession number(s) (GI #2393947) (NCBI Accession #AAC51766.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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(Or you can also download the PDF Manual for complete product instructions).
Intended Uses: This ADAM10 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ADAM10. This ELISA kit for research use only!
Principle of the Assay: ADAM10 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ADAM10 antibody and an ADAM10-HRP conjugate. The assay sample and buffer are incubated together with ADAM10-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADAM10 concentration since ADAM10 from samples and ADAM10-HRP conjugate compete for the anti-ADAM10 antibody binding site. Since the number of sites is limited, as more sites are occupied by ADAM10 from the sample, fewer sites are left to bind ADAM10-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADAM10 concentration in each sample is interpolated from this standard curve.
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive or Sandwich
Detection Range: 250-5000pg/mL
Sensitivity: 1.0pg/mL. Human A Disintegrin And Metalloprotease 10 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. ADAM10 also interacts with the following gene(s): CDH2, CTNNB1, DLL1, EGFR, JAG1, JAG2, NOTCH1, NOTCH2, NOTCH3. Bone, Brain, Connective Tissue, Embryonic Tissue, Intestine, Kidney, Liver, Lung, Placenta, Skin tissues are correlated with this protein. Brain Diseases, Breast Neoplasms, Cardiovascular Diseases, Gastrointestinal Diseases, Inflammation, Kidney Diseases, Lung Diseases, Necrosis, Neoplasm Metastasis, Skin Diseases are some of the diseases may be linked to Human A Disintegrin And Metalloprotease 10 ELISA Kit.