ACE2 blocking peptide product blog
Tags: ACE2; Blocking Peptide; ACE2 blocking peptide; ACE2 (SARS Receptor);
The ACE2 ace2 (Catalog #MBS9225887) is a Blocking Peptide and is intended for research purposes only. The product is available for immediate purchase.General recommendations for using blocking peptides in western blot and immunostaining techniques are described below. The precise conditions should be optimized for a particular assay. Protocol: Peptides are used to block antibody binding to its target. In order to visualize the inhibitory effect of the peptide, they are usually used at 10X to 100X excess compared to antibody molarity. We recommend using a 1X, 10X and 100X excess of peptide first, and then to narrow this range if a more accurate study is required. Calculation for antibodies manufactured at 0.25 mg/ml: Using the antibody at 1:100 dilution as recommended corresponds to 2.5 ug/ml. Estimating the MW of an antibody at 150,000 Da, the final antibody concentration is ca. 17 nM. A peptide of 15 residues long has an average MW of 1650 Da (110 Da multiplied by 15). For an excess of 100X of peptide over the antibody used at 17 nM, a concentration of 1.7 uM or 1.7 uM � 1650 = 2.8 ug/ml, is needed. Since one antibody binds two peptides, 5.6 ug/ml is 100X excess. Important Note: It is very important to mix the antibody with the peptide before incubation with the cell lysate or onto slide. Otherwise, you may not be able to disrupt antibody binding from native target. Researchers should empirically determine the suitability of the ACE2 ace2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The ACE2 ace2 product has the following accession number(s) (GI #71658783) (NCBI Accession #Q9BYF1.2) (Uniprot Accession #Q9BYF1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin- 13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses.
Background:
ACE2 cDNA encodes a deduced 805-amino acid protein containing a potential 17-amino acid N-Terminal signal peptide and a putative 22-amino acid C-Terminal membrane anchor. It also possesses a zinc metalloprotease consensus sequence and a conserved glutamine residue that may function as a third zinc ligand. ACE2 is expressed predominantly in vascular endothelial cells of the heart and kidney. ACE converts angiotensin l to angiotensin ll, ACE2 converts angiotensin l to angiotensin 1-9, which has 9 amino acids. Angiotensin ll is a potent blood vessel constrictor, while angiotensin 1-9 does not impact blood vessels but is cleaved by ACE to a shorter peptide, angiotensin 1-7, which is a blood vessel dilator. Spike proteins of coronaviruses, including the SARS coronavirus. Bind with cellular receptors to mediate infection of target cells. ACE2 binds the S1 domain of the SARS coronavirus S protein. SARS coronavirus replicates efficiently on ACE2-Transfected but not mock-transfected 293T cells. Anti-ACE2 but not anti-ACE1 antibody blocks viral replication on Vero E6 cells. It has been proposed the ACE2 is a functional receptor of SARS coronavirus.