A431+ Pervanadate Lysate lysate product blog
Tags: Lysate; A431+ Pervanadate Lysate; A431+ Pervanadate Lysate lysate;
The A431+ Pervanadate Lysate n/a (Catalog #MBS657374) is a Lysate and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s A431+ Pervanadate Lysate can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).SDS-PAGE/Western blotting 20ml/well., End user should determine optimal quantity for their particular applications and experiments. Researchers should empirically determine the suitability of the A431+ Pervanadate Lysate n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
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Pervanadate is a protein tyrosine phosphatase inhibitor that is commonly used to increase tyrosine phosphorylation in cells. When cells are treated with pervanadate for 30 minutes they undergo significant tyrosine phosphorylation, as shown by western blotting using anti-Phosphotyrosine. Confluent cultures of A431 cells were serum starved overnight. Cells were then either left untreated or treated with pervanadate (1 mM) for 30 minutes at 37 degree C Cells were lysed in 1% SDS, 1.0mM sodium ortho-vanadate, 10mM Tris (pH 7.4) buffer. Protein concentration was determined using the BCA method (Pierce) before diluting to final concentration and buffer.