A431 Cells, EGF-Stimulated cell product blog
Tags: Cell; A431 Cells, EGF-Stimulated; A431 Cells, EGF-Stimulated cell;
The A431 Cells, EGF-Stimulated n/a (Catalog #MBS652596) is a Cell Line produced from Human A431 cells and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s A431 Cells, EGF-Stimulated can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).Dilution: Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for immunoblot analysis. This preparation may be used as a positive control for antibodies. Researchers should empirically determine the suitability of the A431 Cells, EGF-Stimulated n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given cell line. We\'ve tested the A431 Cells, EGF-Stimulated with the following immunoassay(s):
Testing Data (EGF-stimulated A431 cell lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-phosphotyrosine (0.5ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.)
Cellular protein preparation from A431 cells containing phosphorylated proteins, including the EGF receptor.