|The A431+ Calyculin A n/a (Catalog #MBS657302) is a Lysate and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s A431+ Calyculin A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).
SDS-PAGE/Western blotting 20ml/well, End user should determine optimal quantity for their particular applications and experiments. Researchers should empirically determine the suitability of the A431+ Calyculin A n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
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Calyculin An is a serine/threonine phosphatase inhibitor that inhibits the activity of protein phosphatases PP1 and PP2A. Human carcinoma A431 cells treated with calyculin A for 30 minutes can undergo significant threonine phosphorylation, as shown by western blotting using anti-Phospho-Akt (Thr-34), as compared to untreated, control cell lysates.