A-Fodrin IgG/IgA elisa kit product blog
Tags: ELISA Kit; Sheep ELISA Kit; Anti-alpha-Fodrin IgG/IgA; A-Fodrin IgG/IgA elisa kit; A-Fodrin IgG/IgA;
The Sheep A-Fodrin IgG/IgA n/a (Catalog #MBS737552) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS737552 ELISA Kit recognizes Sheep A-Fodrin IgG/IgA.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Sheep Anti-alpha-Fodrin IgG/IgA ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: A-Fodrin IgG/IgA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-A-Fodrin IgG/IgA antibody and an A-Fodrin IgG/IgA-HRP conjugate. The assay sample and buffer are incubated together with A-Fodrin IgG/IgA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the A-Fodrin IgG/IgA concentration since A-Fodrin IgG/IgA from samples and A-Fodrin IgG/IgA-HRP conjugate compete for the anti-A-Fodrin IgG/IgA antibody binding site. Since the number of sites is limited, as more sites are occupied by A-Fodrin IgG/IgA from the sample, fewer sites are left to bind A-Fodrin IgG/IgA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The A-Fodrin IgG/IgA concentration in each sample is interpolated from this standard curve.
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Sensitivity: 0.1 U/ml. Intended Uses: This A-Fodrin IgG/IgA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep A-Fodrin IgG/IgA. This ELISA kit for research use only, not for therapeutic applications!. Sheep Anti-alpha-Fodrin IgG/IgA ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.