4 NT elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; 4 Nitrotyrosine; 4 NT elisa kit; 4 NT;
The Human 4 NT n/a (Catalog #MBS733050) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS733050 ELISA Kit recognizes Human 4 NT.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human 4 Nitrotyrosine ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Intended Uses: This 4-NT ELISA kit is intended Laboratory for research use only.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of 4-NT in the sample, this 4-NT ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus 4-NT concentration. The concentration of 4-NT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: This 4-NT enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for 4-NT. Standards or samples are then added to the microtiter plate wells and 4-NT if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of 4-NT present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for 4-NT are added to each well to "sandwich" the 4-NT immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, An and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that contain 4-NT and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Detection Range: 100-2500pg/mL
Sensitivity: 1.0pg/mL. Human 4 Nitrotyrosine ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.