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3-NT elisa kit product blog

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Posted on 2015-07-14 10:44:22 by mybiosource_staff
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Tags: ELISA Kit; Rat ELISA Kit; 3-NT elisa kit; 3-NT; 3-nitrotyrosine (3-NT);
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The Rat 3-NT n/a (Catalog #MBS936297) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS936297 ELISA Kit recognizes Rat (Rattus, General) 3-NT.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Rat 3-nitrotyrosine, 3-NT ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) 3-NT.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for 3-NT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any 3-NT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for 3-NT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of 3-NT bound in the initial step. The color development is stopped and the intensity of the color is measured.

Samples: Serum, plasma
Assay Type: Sandwich
Detection Range: 0.156 ng/ml-10 ng/ml
Sensitivity: 0.039 ng/ml. Precision: Intra-assay Precision (Precision within an assay) : CV%<8% Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays) : CV%<10% Three samples of known concentration were tested in twenty assays to assess. Rat 3-nitrotyrosine, 3-NT ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
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