|The Guinea Pig 3-NT n/a (Catalog #MBS732193) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS732193 ELISA Kit recognizes Guinea Pig (Cavia, Cavy, Porcellus, General) 3-NT.
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for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Guinea pig 3-Nitrotyrosine ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the assay: 3-NT ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-3-NT antibody and an 3-NT-HRP conjugate. The assay sample and buffer are incubated together with 3-NT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the 3-NT concentration since 3-NT from samples and 3-NT-HRP conjugate compete for the anti-3-NT antibody binding site. Since the number of sites is limited, as more sites are occupied by 3-NT from the sample, fewer sites are left to bind 3-NT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 3-NT concentration in each sample is interpolated from this standard curve.
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Sensitivity: 1.0 pg/mL. Intended Uses: This 3-NT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig 3-NT. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Guinea pig 3-Nitrotyrosine ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.