11-Deoxycortisol elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; 11-Deoxycortisol; 11-Deoxycortisol elisa kit;
The Human 11-Deoxycortisol n/a (Catalog #MBS282369) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS282369 ELISA Kit recognizes Human 11-Deoxycortisol.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Intended Uses: For the quantitative detection of Human 11-Deoxycortisol concentration in serum, plasma and other biological fluids.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 11-Deoxycortisol. Standards or samples are added to the appropriate microtiter plate wells with biotin-conjugated 11-Deoxycortisol. A competitive inhibition reaction is launched between 11-Deoxycortisol (Standards or samples) and biotin-conjugated 11-Deoxycortisol with the pre-coated antibody specific for 11-Deoxycortisol. The more amount of 11-Deoxycortisol in samples, the less antibody bound by biotin-conjugated 11-Deoxycortisol. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of 11-Deoxycortisol in the sample. The color development is stopped and the intensity of the color is measured.
Samples: Serum, plasma and other biological fluids
Assay Type: Quantitative Competitive
Detection Range: 1.25 pmol/mL - 20 pmol/mL
Sensitivity: 0.625 pmol/mL. Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV(%) = SD/meanX100. Inter-Assay: CV<12%. Human 11-Deoxycortisol ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.