1, 25 DHVD3 elisa kit product blog
Tags: ELISA Kit; Rat ELISA Kit; 1, 25-dihydroxyvitamin D3; 1, 25 DHVD3 elisa kit; 1, 25 DHVD3;
The Rat 1, 25 DHVD3 n/a (Catalog #MBS704153) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS704153 ELISA Kit recognizes Rat 1, 25 DHVD3.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Rat 1, 25-dihydroxyvitamin D3, 1, 25 DHVD3 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated 1,25 DHVD3 Hapten. The competitive inhibition reaction is launched between with HRP-conjugated 1,25 DHVD3 Hapten and 1,25 DHVD3 in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of 1,25 DHVD3 in the sample. The color development is stopped and the intensity of the color is measured.
Samples: Serum
Assay Type: Quantitative Competitive
Detection Range: 1000 fmol/L-5000 fmol/L
Sensitivity: <250 fmol/L. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<10%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess. Rat 1, 25-dihydroxyvitamin D3, 1, 25 DHVD3 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.